Diuron causes sinking retardation and physiochemical alteration in marine diatoms Thalassiosira pseudonana and Skeletonema marinoi-dohrnii complex

• Published in: Chemosphere (Oxford) Vol. 175; pp. 200 - 209
• Main Authors: Khanam, Mst Ruhina Margia, Shimasaki, Yohei, Hosain, Md Zahangir, Mukai, Koki, Tsuyama, Michito, Qiu, Xuchun, Tasmin, Rumana, Goto, Hiroshi, Oshima, Yuji
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2017
• Subjects: Diatoms; Diatoms - drug effects; Diatoms - physiology; Diuron; Diuron - toxicity; Herbicides - toxicity; Photosynthesis - drug effects; Sinking rate; Skeletonema marinoi-dohrnii complex; Thalassiosira pseudonana; Water Pollutants, Chemical - toxicity; Thalassiosira pseudonana; Diuron; Sinking rate; Diatoms; Skeletonema marinoi-dohrnii complex
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2017.02.054

The present research investigated the effect of diuron on sinking rate and the physiochemical changes in two marine diatoms, Thalassiosira pseudonana (single-celled species) and Skeletonema marinoi-dohrnii complex (chain-forming species). The results revealed that the sinking rate of both diatoms exposed to diuron at a level of 50% effective concentration for growth (EC50) decreased significantly compared with the control. Photosynthetic performance (Fv/Fm and PIABS) of both diatoms also decreased significantly with diuron exposure. The number of cells per chain in S. marinoi-dohrnii decreased significantly with diuron treatment, but T. pseudonana cell diameter remained stable. Neutral lipid concentration per cell was significantly higher compared with control at 72 h in both diatom species exposed to EC50 level diuron. And water-soluble protein concentration per cell at 72 h was lower than control in the T. pseudonana EC50 group only. These biochemical changes may decrease specific gravity of cells and seems to cause a decreased sinking rate in diatoms. The positive significant correlation between the numbers of cells per chain and sinking rate in S. marinoi-dohrnii indicated that chain length is also an important factor in sinking rate regulation for chain-forming diatoms. Thus, our present study suggested that suppression of photosynthetic performance and the resultant physiochemical changes induced the decreased sinking rate that may inhibit the normal survival strategy (avoidance from the surface layer where strong light either causes photo-inhibition or interrupts resting cell formation). Therefore, the use of antifouling agents should be considered for the sustainable marine environment. •Diuron retarded diatom sinking rate.•Decreased sinking rate might be caused by physiochemical alteration.•Sinking retardation may cause ecological disruption in diatoms.•Sinking of diatoms must be considered for the risk evaluation of pollutants.