Improved method for the determination of aspirin and its metabolites in biological fluids by high-performance liquid chromatography: applications to human and animal studies

An improved method has been developed for the determination of acetylsalicylic acid, salicylic acid, gentisic acid, and salicyluric acid in plasma and urine of rabbits and man. Samples are extracted with dichloromethane containing mephenytoin as an internal standard, the solvent is evaporated under...

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Bibliographic Details
Published inJournal of chromatography Vol. 311; no. 2; pp. 301 - 309
Main Authors Mays, D.C., Sharp, D.E., Beach, C.A., Kershaw, R.A., Bianchine, J.R., Gerber, N.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.01.1984
Subjects
Animals
Aspirin - blood
Aspirin - urine
Biological Availability
Chromatography, High Pressure Liquid - methods
Gentisates
Hippurates - blood
Hippurates - urine
Humans
Hydroxybenzoates - blood
Hydroxybenzoates - urine
Kinetics
Rabbits
Rats
Salicylates - blood
Salicylates - urine
Salicylic Acid
Species Specificity
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Summary:An improved method has been developed for the determination of acetylsalicylic acid, salicylic acid, gentisic acid, and salicyluric acid in plasma and urine of rabbits and man. Samples are extracted with dichloromethane containing mephenytoin as an internal standard, the solvent is evaporated under reduced pressure, the residue reconstituted and analyzed by high-performance liquid chromatography. Extraction efficiencies, linearity and assay precision were determined. This method has been applied to human bioavailability studies and the data are presented.
ISSN:0378-4347
DOI:10.1016/S0378-4347(00)84723-4