Involvement of ligninolytic enzymes of Myceliophthora vellerea HQ871747 in decolorization and complete mineralization of Reactive Blue 220

• Published in: Chemical engineering journal (Lausanne, Switzerland : 1996) Vol. 233; pp. 98 - 108
• Main Authors: Patel, Vipul R., Bhatt, Nikhil S., B̀Bhatt, H.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2013
• Subjects: benzene; Biodegradation; chemical engineering; decayed wood; decolorization; dyes; GC–MS; HPLC; Immobilization; laccase; mineralization; Myceliophthora; Myceliophthora vellerea HQ871747; NMR; phylogeny; physicochemical properties; plant growth; soil bacteria; sulfonic acid; wastewater; Biodegradation; Immobilization; Myceliophthora vellerea HQ871747; NMR; GC–MS; HPLC
• ISSN: 1385-8947; 1873-3212
• DOI: 10.1016/j.cej.2013.07.110

•Good resistance of Myceliophthora vellerea HQ871747 towards wide array of dyes.•Immobilized packed bed reactor was operated up to 1600mgL−1 with 2% NaCl concentration.•Characterization and purification lignolytic enzymes MnP and laccase of Myceliophthora vellerea HQ871747.•Degradation monitored by higher spectroscopy, no metabolites detected in the treated water.•Toxicity of dyes and their metabolites were assessed on plant/bacterial growth. Myceliophthora vellerea HQ871747 was isolated from decayed wood sample and was subjected to phylogenetic analysis by using basidiomycete-specific ITS primers. Preferable physicochemical parameters were identified to achieve maximum enzyme and decolorization efficiency. M. vellerea HQ871747 tolerated 20gL−1 NaCl concentration without decrease in average rate of decolorization and showed more than 50% decolorization up to 7th cycle of repetitive dye addition. It is also able to degrade mixture of various reactive, acidic, direct and disperse dyes. In immobilized packed-bed reactor 29–65mgL−1h−1 decolorization rate was achieved with >80% COD removal, 154 and 75UL−1MnP and laccase activity respectively. Purified MnP and laccase were monomeric protein with corresponding 37 and 25kDa size on the SDS–PAGE. In this study it is proposed that the asymmetric cleavage of RB220 and HPLC, NMR and GC–MS confirmed formation of 2-diazo benzene sulfonic acid, naphthalene-2-sulfonic acid and 4-(4-diazenyl-5-oxo-4, 5-dihydro-1H-pyrazol-1-yl) benzene sulfonic acid. Treated wastewater was found to stimulate the growth of selected soil bacteria as well as plant growth.