A re-assessment of styrene-induced clastogenicity in mice in a subacute inhalation study

• Published in: Archives of toxicology Vol. 77; no. 1; pp. 56 - 61
• Main Authors: ENGELHARDT, Günter, GAMER, Armin, VODICKA, Pavel, BARTA, Ivo, HOFFMANN, Hans-Dieter, VEENSTRA, Gauke
• Format: Journal Article
• Language: English
• Published: Berlin Springer 2003
• Subjects: Administration, Inhalation; Animals; Biological and medical sciences; Bone Marrow Cells - drug effects; Chemical and industrial products toxicology. Toxic occupational diseases; Dose-Response Relationship, Drug; Erythrocytes - drug effects; Male; Medical sciences; Mice; Micronuclei, Chromosome-Defective - drug effects; Micronucleus Tests; Mutagenesis; Mutagens - administration & dosage; Mutagens - toxicity; Single-Blind Method; Solvents; Styrene - administration & dosage; Styrene - toxicity; Toxicology; Chromosomal aberration; Micronucleus test; Toxicity; Rodentia; Inhalation; Micronucleus assay· Clastogenicity; Organic solvent; Vertebrata; Chromosome break; Mammalia; Mouse; Animal; Micronucleus; Styrene; Cytogenetics; Bone marrow; Subacute
• ISSN: 0340-5761; 1432-0738
• DOI: 10.1007/s00204-002-0406-y

To date, a number of in vivo cytogenetic assays have studied the clastogenicity (chromosome aberrations, micronuclei formation) in bone marrow of rodents exposed to styrene by various routes. The majority of all these cytogenetic experiments yielded negative findings (Scott and Preston, Mutat Res 318:175-203, 1994). Recently published data from a micronucleus test in mice exposed via inhalation for up to 21 days showed some positive response, but was not fully conclusive (Vodicka et al., Chem Biol Interact 137:213-227, 2001). Since this exposure regimen has considerable relevance for workplace exposure, the present study was performed to further elucidate these findings. NMRI mice were exposed by whole body inhalation to styrene concentrations of 750 mg/m3 and 1,500 mg/m3 for 1, 3, 7, 14 and 21 consecutive days (6 h/day). Animals were killed directly after exposure and bone marrow was sampled for analysis of micronucleus induction. Under the experimental conditions used in the present investigation, there was no evidence of clastogenicity at any concentration or exposure interval.