The reduction effect and mechanism of Deinococcus radiodurans transformed dsrA gene to uranyl ions

• Published in: Journal of radioanalytical and nuclear chemistry Vol. 330; no. 3; pp. 1075 - 1090
• Main Authors: Cheng, Conghui, Xie, Jingxi, Zhu, Qiqi, Chen, Luyao, Guo, Kexin, Li, Shanshan, He, Shuya, Xiao, Fangzhu
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.12.2021; Springer; Springer Nature B.V
• Subjects: Bacteria; Chemistry; Chemistry and Materials Science; Diagnostic Radiology; Genes; Genetic engineering; Genetically modified organisms; Hadrons; Heavy Ions; Inorganic Chemistry; Ionizing radiation; Malondialdehyde; Nuclear Chemistry; Nuclear energy; Nuclear Physics; Oxidases; Physical Chemistry; Radiation; Radiation tolerance; Uranium; Oxidative stress; Uranium; Reduction gene; Reduction enrichment
• ISSN: 0236-5731; 1588-2780
• DOI: 10.1007/s10967-021-08038-7

Deinococcus radiodurans (DR) is highly resistant to ionizing radiation. This study aims to convert dsrA gene into DR to construct radiation-resistant genetically engineered bacteria (Deino- dsrA ) with high reducibility, so as to enhance the reducing and enrichment ability of DR. Methods: the recombinant vector pRADK- dsrA was extracted and transformed into DR. 1. Radiation resistant gene engineering bacteria containing dsrA gene were constructed successfully. 2. In the most favorable conditions,contributing to approximately 92.45% U (VI) was removed. 3. Autioxidant enzyme activities in Deino- dsrA is higher than in Deino-pRADK, excepting the content of malondialdehyde. The uranium enrichment ability of the Deino- dsrA is better than the wild DR, and dsrA gene can increase its antioxidation capability by increasing the activity of oxidase.