Keratinocytes Immortalized by Human Papillomavirus-18 Exhibit Alterations Dependent upon Host Genetic Background and Complexity of Viral Genes Transfected

• Published in: Pathobiology (Basel) Vol. 62; no. 1; pp. 43 - 52
• Main Authors: Pei, Xu Fang, Qin, Nai Geng, Meck, Jeanne M., Schlegel, Richard
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Karger 01.01.1994
• Subjects: Biological and medical sciences; Cell Line; DNA, Viral - analysis; DNA, Viral - genetics; DNA-Binding Proteins; Fundamental and applied biological sciences. Psychology; Genes, Viral; Genome, Viral; Human Papillomavirus-18; Humans; Infant, Newborn; Karyotyping; Keratinocytes - cytology; Male; Microbiology; Oncogene Proteins, Viral - genetics; Papillomaviridae - genetics; Papillomaviridae - physiology; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Transfection; Virology; Human papillomaviruses; Keratinocytes; Cell immortalization; Cell culture; Growth; Exploration; Host agent relation; Papovaviridae; Malignant tumor; Host virus relation; Human papillomavirus 18; Infection; Papillomavirus; Virus; Northern blotting; Human papillomavirus 16; Viral disease; Morphological analysis; Established cell line; Cytogenetics; Keratinocyte; Molecular biology; Genome; Southern blotting; Karyotype
• ISSN: 1015-2008; 1423-0291
• DOI: 10.1159/000163876

Keratinocytes immortalized by the human papillomaviruses (HPVs) vary in cell morphology, growth properties, resistance to inducers of terminal differentiation, and karyotype. To determine the contribution of the host cell genetic background and the HPV genes to these cellular alterations, we have generated and characterized 6 human keratinocyte lines from two different newborn foreskins (A and B) using either the full-length HPV 18 genome or the isolated HPVl 8 E6/E7 genes. The growth properties of the immortalized lines were found to correlate with the complexity of HPV genes present in the transfected vector. Interestingly, cell lines established from foreskin A revealed common chromosomal alterations regardless of the HPV construct utilized for immortalization, and these karyotypic changes differed from those observed in cell lines established from foreskin B, which exhibited their own characteristic aneuploid profile. Thus, chromosomal alterations of HPV-immortalized cells are in part determined by the host genetic background.