Analysis of Salmonella lipopolysaccharides by sodium deoxycholate-polyacrylamide gel electrophoresis

Lipopolysaccharides (LPSs) were analyzed by polyacrylamide gel electrophoresis (PAGE) in the presence of sodium deoxycholate (DOC) or sodium dodecyl sulphate (SDS) and the results obtained compared. Two-dimensional PAGE of the S form of LPS revealed that, with SDS, bands which appeared to be single...

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Bibliographic Details
Published inJournal of chromatography Vol. 450; no. 3; p. 381
Main Authors Komuro, T, Galanos, C
Format Journal Article
LanguageEnglish
Published Netherlands 1988
Subjects
Deoxycholic Acid
Electrophoresis, Polyacrylamide Gel
Lipopolysaccharides - analysis
Molecular Weight
Salmonella typhimurium - analysis
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Summary:Lipopolysaccharides (LPSs) were analyzed by polyacrylamide gel electrophoresis (PAGE) in the presence of sodium deoxycholate (DOC) or sodium dodecyl sulphate (SDS) and the results obtained compared. Two-dimensional PAGE of the S form of LPS revealed that, with SDS, bands which appeared to be single after the one-dimensional experiment were resolved into several bands after two-dimensional electrophoresis. In the presence of DOC, however, a diagonal of single bands was obtained in one-dimensional electrophoresis indicating optimum resolution. The high quality of resolution by DOC-PAGE was constant for amounts of LPS up to 20 micrograms. Finally, DOC-PAGE does not require boiling of the samples in DOC, which may be an advantage over SDS-PAGE.
DOI:10.1016/S0021-9673(01)83593-7