A Role for the GAL11 Protein in Pheromone-Induced Transcription in Saccharomyces cerevisiae

• Published in: Biochemical and biophysical research communications Vol. 212; no. 3; pp. 854 - 860
• Main Authors: Dolan, J.W., Gatlin, J.E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 26.07.1995
• Subjects: ACCOUPLEMENT; Base Sequence; COPULA; DNA Primers - genetics; DNA, Fungal - genetics; EXPRESION GENICA; EXPRESSION DES GENES; FEROMONAS; Fungal Proteins - genetics; GENE; GENES; Genes, Fungal; Genes, Mating Type, Fungal; Genes, Regulator; Mediator Complex; Molecular Sequence Data; MUTACION; MUTATION; Phenotype; PHEROMONE; Pheromones - pharmacology; Promoter Regions, Genetic; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins; Trans-Activators; Transcription Factors - genetics; Transcription, Genetic - drug effects
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1995.2047

The product of the SaccharomycescerevisiaeGAL11 gene, Gal11p, is required for the proper expression of a wide range of genes including many mating-type-specific genes. In this study Gal11p is shown to have a role in the induction of transcription by pheromone. Basal transcription of an a-specific gene is unaffected by loss of Gal11p but pheromone treatment fails to increase transcription. A haploid-specific gene is shown to retain pheromone-inducibility in the absence of Gal11p but the extent of induction is drastically reduced. Evidence is also presented that suggests the existence of strain differences that can alter the phenotype of gal11 mutants.