Human miR-31 targets radixin and inhibits migration and invasion of glioma cells

• Published in: Oncology reports Vol. 27; no. 3; pp. 700 - 706
• Main Authors: DASONG HUA, DONG DING, XU HAN, WEIYI ZHANG, NA ZHAO, FOLTZ, Gregory, QING LAN, QIANG HUANG, BIAOYANG LIN
• Format: Journal Article
• Language: English
• Published: Athens Spandidos 01.03.2012
• Subjects: Base Sequence; Biological and medical sciences; Brain Neoplasms - genetics; Brain Neoplasms - pathology; Cell Line, Tumor; Cell Movement - genetics; Cytoskeletal Proteins - genetics; Down-Regulation; Gene Expression Profiling - methods; Gene Expression Regulation, Neoplastic; Glioblastoma - genetics; Glioblastoma - pathology; Humans; Medical sciences; Membrane Proteins - genetics; MicroRNAs - biosynthesis; MicroRNAs - genetics; Neoplasm Invasiveness; Neurology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Tumors; Tumors of the nervous system. Phacomatoses; Human; Nervous system diseases; Targeting; Malignant tumor; Metastasis; Invasion; Cancerology; Glioma; radixin; Central nervous system disease; miR-31; Inhibitor; Cell migration; Cancer
• ISSN: 1021-335X; 1791-2431
• DOI: 10.3892/or.2011.1555

MicroRNAs (miRNAs) are a novel group of short RNAs, about 20‑22 nucleotide in length, that regulate gene expression in a post-transcriptional manner by affecting the stability or translation of mRNAs and play important roles in many biological processes. Many microRNAs have been implicated in glioblastoma. miR-31 is dysregulated in several types of cancer including colon, breast, prostate, gastric and lung cancers. However, the expression and role of miR-31 in glioblastoma are still unclear. In this study, we performed real-time reverse transcriptase polymerase chain reaction (RT-PCR) assays on 10 glioblastoma and 7 normal brain tissues. We found that miR-31 is down-regulated in glioblastoma compared with normal brain tissues. Ectopic expression of miR-31 inhibited migration and invasion ability of U251 glioma cells. Expression profiling analysis revealed that miR-31 affected the cell migration and motility process by regulating migration and invasion related genes. Finally, we demonstrated that miR-31 targeted radixin predominantly via inhibition of protein translation instead of degradation of mRNA.