Determination of catecholamines in human serum by micro high-performance liquid chromatography with micro precolumn and dual electrochemical detection

A dual electrochemical detector having two working electrodes (anode and cathode) in parallel—opposed configuration suitable for micro high-performance liquid chromatography was developed for the selective and sensitive detection of catecholamines on the basis of their electrochemical reversibility...

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Bibliographic Details
Published inJournal of chromatography Vol. 275; no. 2; pp. 271 - 281
Main Authors Goto, Masashi, Zou, Gongwei, Ishii, Daido
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.01.1983
Subjects
Catecholamines - blood
Chromatography, High Pressure Liquid - instrumentation
Electrochemistry - instrumentation
Humans
Ultrafiltration
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Summary:A dual electrochemical detector having two working electrodes (anode and cathode) in parallel—opposed configuration suitable for micro high-performance liquid chromatography was developed for the selective and sensitive detection of catecholamines on the basis of their electrochemical reversibility and catalytic amplification by recycling oxidation and re-reduction. The micro high-performance liquid chromatographic system with a micro alumina precolumn for enriching catecholamines and the dual electrochemical detector in parallel—opposed configuration was successfully utilized for the determination of catecholamines in healthy human serum injected directly after ultrafiltration.
ISSN:0378-4347
DOI:10.1016/S0378-4347(00)84374-1