Metal-organic frameworks-based biosensor for microRNA detection in prostate cancer cell lines

• Published in: RSC advances Vol. 12; no. 54; pp. 3517 - 3518
• Main Authors: Ahmadi Najafabadi, Milad, Yousefi, Fatemeh, Rasaee, Mohammad J, Soleimani, Masoud, Kazemzad, Mahmood
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 06.12.2022; The Royal Society of Chemistry
• Subjects: Biosensors; Chemistry; Dyes; Fluorescence; Folic acid; Metal-organic frameworks; Ribonucleic acid; RNA
• ISSN: 2046-2069; 2046-2069
• DOI: 10.1039/d2ra04959g

In this research, a novel dye-labeled probe (FAM-Probe) based on a nano metal-organic framework (NMOF) functionalized with folate (NMOF-FA) was prepared and applied as a fluorescent sensing platform for the recognition of intracellular microRNA (miRNA-21) in DU145, PC3, and LNCaP cancer cells. The NMOF-FA can be easily assembled with a dye-labeled miR-21 probe (FAM-Probe21), causing an efficient fluorescence quenching of fluorescence of FAM fluorophore. The probe can be specifically catch up by cancerous cells through targeting their folate receptor by folic acid on the FAM-Probe21-NMOF-FA complex. Upon the interaction of the FAM-Probe21-NMOF-FA with complementary miRNA (miR-21), the fluorescence intensity can be recovered, providing a specific system to detect miRNAs in prostate cancer cells. We used the proposed probe for cell-specific intracellular miRNA-21 sensing, following the alteration expression level of miRNA-21 inside living cells. Thus, the FAM-Probe21-NMOF-FA complex can be used as a new miRNA sensing method in biomedicine studies. A novel FAM-Probe based on a nano metal-organic framework was functionalized with folate and applied to detect the intracellular miR-21 in prostate cancer cells. Upon the interaction of the probe with complementary miRNA, the fluorescence intensity can be recovered.