Effects of Modification of Amino Groups with Poly(Ethylene Glycol) on a Recombinant Uricase from Bacillus fastidiosus

• Published in: Bioscience, biotechnology, and biochemistry Vol. 74; no. 6; pp. 1298 - 1301
• Main Authors: ZHANG, Chun, YANG, Xiaolan, FENG, Juan, YUAN, Yonghua, LI, Xiang, BU, Youquan, XIE, Yanling, YUAN, Huidong, LIAO, Fei
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 23.06.2010; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Animals; Bacillus - enzymology; Biological and medical sciences; Enzyme Activation; Enzyme Stability; Fundamental and applied biological sciences. Psychology; Half-Life; Hydrogen-Ion Concentration; hyperuricemia; Male; PEG modification; Polyethylene Glycols - chemistry; Rats; Recombinant Proteins - blood; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism; Structure-Activity Relationship; Temperature; thermo-stability; Urate Oxidase - blood; Urate Oxidase - chemistry; Urate Oxidase - metabolism; uricase; Stability; PEG modification; Enzyme; Urate oxidase; uricase; Bacillaceae; Ethylene oxide polymer; Hyperuricemia; Bacillales; Bacillus; Bacteria; Oxidoreductases; thermo-stability
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.100080

After modification with monomethoxyl-poly(ethylene glycol)-5000, a recombinant intracellular uricase from Bacillus fastidiosus ATCC 29604 showed residual activity of about 65%, a thermo-inactivation half-life >85 h, a circulating half-life about 20 h in rats in vivo, consistent effects of common cations, and consistent optima for reaction temperature and pH. Thus, this uricase can be formulated via modification with monomethoxyl-poly(ethylene glycol).