Simultaneous two-wavelength transmission quantitative phase microscopy with a color camera

We present a quantitative phase microscopy method that uses a Bayer mosaic color camera to simultaneously acquire off-axis interferograms in transmission mode at two distinct wavelengths. Wrapped phase information is processed using a two-wavelength algorithm to extend the range of the optical path...

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Bibliographic Details
Published inOptics letters Vol. 35; no. 15; p. 2612
Main Authors Rinehart, Matthew T, Shaked, Natan T, Jenness, Nathan J, Clark, Robert L, Wax, Adam
Format Journal Article
LanguageEnglish
Published United States 01.08.2010
Subjects
Algorithms
Animals
Humans
Image Processing, Computer-Assisted - methods
Microfluidics
Microscopy - methods
Microscopy, Electron, Scanning - methods
Optics and Photonics
Refractometry
Reproducibility of Results
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Summary:We present a quantitative phase microscopy method that uses a Bayer mosaic color camera to simultaneously acquire off-axis interferograms in transmission mode at two distinct wavelengths. Wrapped phase information is processed using a two-wavelength algorithm to extend the range of the optical path delay measurements that can be detected using a single temporal acquisition. We experimentally demonstrate this technique by acquiring the phase profiles of optically clear microstructures without 2pi ambiguities. In addition, the phase noise contribution arising from spectral channel crosstalk on the color camera is quantified.
ISSN:1539-4794
DOI:10.1364/OL.35.002612