Preliminary Classification of the ABC Transporter Family in Betula halophila and Expression Patterns in Response to Exogenous Phytohormones and Abiotic Stresses

• Published in: Forests Vol. 10; no. 9; p. 722
• Main Authors: An, Linjun, Ma, Qing, Du, Jinxia, Yu, Miao, Li, Fangrui, Luan, Jiayu, Jiang, Jing, Li, Huiyu
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.09.2019
• Subjects: ABC transporter; ABC transporters; Abiotic stress; abiotic stress response; Abscisic acid; ATP-binding cassette transporter; Betula halophila; Exposure; Gene expression; Genes; hormone response; Hormones; Localization; Peptides; Phylogenetics; Phylogeny; Phytohormones; Plant cells; Plant resistance; Polyethylene glycol; Polymerase chain reaction; Proteins; Salt; Seeds; Thermal cycling; Transcription; Transcriptomes; Transportation systems; Beijing China; China
• ISSN: 1999-4907; 1999-4907
• DOI: 10.3390/f10090722

ATP-binding cassette (ABC) transporters comprise a transport system superfamily which is ubiquitous in eukaryotic and prokaryotic cells. In plants, ABC transporters play important roles in hormone transport and stress tolerance. In this study, 15 BhABC transporters encoded by genes identified from the transcriptome of Betula halophila were categorized into four subfamilies (ABCB, ABCF, ABCG, and ABCI) using structural domain and phylogenetic analyses. Upon B. halophila exposure to exogenous phytohormones and abiotic stressors, gene expression patterns and transcriptional responses for each subfamily of genes were obtained using semi-quantitative RT-PCR analysis. The results demonstrated that expression of most genes belonging to ABCB and ABCG subfamilies changed in response to exogenous phytohormone exposures and abiotic stress. These results suggest that BhABC genes may participate in hormone transport and that their expression may be influenced by ABA-dependent signaling pathways involved in abiotic stress responses to various stressors.