Real-time and label-free detection of biomolecular interactions by oblique-incidence reflectivity difference method

• Published in: Chinese physics B Vol. 20; no. 1; pp. 272 - 275
• Main Author: 王旭 陆珩 戴俊 温娟 原昆 吕惠宾 金奎娟 周岳亮 杨国桢
• Format: Journal Article
• Language: English
• Published: IOP Publishing 2011
• Subjects: 动力学过程; 反射率; 实时检测; 寡核苷酸; 无标记; 杂交组合; 环氧基团; 生物分子相互作用
• ISSN: 1674-1056; 2058-3834
• DOI: 10.1088/1674-1056/20/1/010704

We successfully conduct the label-free and real-time detection of the interactions between epoxy groups and rabbit IgG and 5＇ CTT CAG GTC ATG AGC CTG AT 3＇ oligonucleotide, and between the hybridization of 5＇ CTT CAG GTC ATG AGC CTG AT 3＇ and its complementary 3＇ GAA GTC CAC TAC TCG GAC TA 5＇ oligonucleotide, by the oblique-incidence reflectivity difference （OI-RD） method. The dynamic curves of OI-RD signals, corresponding to the kinetic processes of biomolecular combination or hybridization, are acquired. In our case, the combination of epoxy groups with rabbit IgG and 5＇ CTT CAC CTC ATG AGC CTG AT 3＇ oligonucleotide need almost one and a half hours and about two hundred seconds, respectively; and the hybridization of the two oligonucleotides needs about five hundred seconds. The experimental results show that the OI-RD is a promising method for the real-time and label-free detection of biomolecular interactions.