High-performance ion-exchange chromatography of oligoribonucleotides using linear and hyperbolic salt gradients

• Published in: Journal of chromatography Vol. 465; no. 2; p. 325
• Main Authors: Tsygankov AYu, Motorin YuA, Wolfson, A D, Kirpotin, D B, Orlovsky, A F
• Format: Journal Article
• Language: English
• Published: Netherlands 1989
• Subjects: Chromatography, High Pressure Liquid; Oligoribonucleotides - analysis; Poly A - analysis; Poly U - analysis; RNA, Transfer - analysis
• DOI: 10.1016/S0021-9673(01)92670-6

A method for separation and chain length determination of oligo- and polynucleotides by high-performance anion-exchange chromatography was developed, which allows resolution of individual fragments according to their chain length n, up to n approximately 10 by linear gradient of sodium chloride and up to n approximately 30 by an hyperbolic gradient of this salt. The hyperbolic relationship between n and the salt concentration at which elution of the fragment occurs allows determination of the degree of polymerization of oligo- and polynucleotides with unknown n. The method proposed can be used for estimation of the effective charge of nucleic acids with complex structure.