A canine model of acute coronary artery thrombosis for the evaluation of reperfusion strategies

• Published in: Cardiology Vol. 84; no. 1; p. 1
• Main Authors: Gurbel, P A, MacCord, C S, Anderson, R D, Scott, H, Atar, D, Mergner, W, Herzog, W R
• Format: Journal Article
• Language: English
• Published: Switzerland 1994
• Subjects: Animals; Blood Platelets - pathology; Coronary Thrombosis - pathology; Coronary Thrombosis - therapy; Coronary Vessels - pathology; Disease Models, Animal; Dogs; Endothelium, Vascular - pathology; Male; Microscopy, Electron; Thrombolytic Therapy; Tissue Plasminogen Activator - administration & dosage
• ISSN: 0008-6312
• DOI: 10.1159/000176322

A model of coronary artery thrombosis which: (1) provides a stable thrombus; (2) incorporates intimal injury; (3) has a low mortality rate; (4) responds predictably to thrombolytic therapy, and (5) is technically simple, was developed. Intimal injury was produced proximal to a critical stenosis and followed by the infusion of a blood and thrombin mixture into the injured segment. After thrombus formation flow remained absent in all control animals (n = 7). Microscopy showed intimal injury and coronary thrombosis with platelets adherent to the subendothelium. In animals treated with tissue plasminogen activator (n = 7) flow returned to > 60% of baseline at 20.2 +/- 7.7 min and was cyclical. Mortality and complications were infrequent. This model is useful in investigations of reperfusion therapy.