FT-IR study of heterologous protein expression in recombinant Escherichia coli strains

Two recombinant Escherichia coli strains expressing different levels of an interferon fusion protein as inclusion bodies have been studied by Fourier transform infrared (FT-IR) microspectroscopy. A marker band at 1628 cm −1 allowed monitoring of the protein expression by direct analysis of cell pell...

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Published inBiochimica et biophysica acta Vol. 1624; no. 1; pp. 6 - 10
Main Authors Ami, Diletta, Bonecchi, Loredana, Calı&#x0300, Simona, Orsini, Gaetano, Tonon, Giancarlo, Doglia, Silvia Maria
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 05.12.2003
Subjects
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Expression of heterologous protein
FT-IR microspectroscopy
Inclusion Bodies - chemistry
Inclusion Bodies - metabolism
Inclusion body
Interferon alpha
Interferon alpha-2
Interferon-alpha - analysis
Interferon-alpha - biosynthesis
Recombinant Fusion Proteins - analysis
Recombinant Fusion Proteins - biosynthesis
Recombinant Proteins
Spectroscopy, Fourier Transform Infrared
Expression of heterologous protein
FT-IR microspectroscopy
Inclusion body
Escherichia coli
Interferon alpha
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Summary:Two recombinant Escherichia coli strains expressing different levels of an interferon fusion protein as inclusion bodies have been studied by Fourier transform infrared (FT-IR) microspectroscopy. A marker band at 1628 cm −1 allowed monitoring of the protein expression by direct analysis of cell pellets in a rapid, non-invasive and quantitative way. The results demonstrate that FT-IR microspectroscopy is a technique of potential biotechnological interest for studying inclusion body formation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/j.bbagen.2003.09.008