Microelectrophoresis of brain and pineal proteins in polyacrylamide gel

• Published in: Analytical biochemistry Vol. 9; no. 1; pp. 9 - 20
• Main Authors: Pun, John Y., Lombrozo, Leon
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.1964
• Subjects: Acrylic Resins; Basal Ganglia; Brain; Cerebral Cortex; Electrophoresis; Ganglia; Hypothalamus; Microchemistry; Nerve Tissue Proteins; Old Medline; Pineal Gland; Proteins; Rats; ELECTROPHORESIS; EXPERIMENTAL LAB STUDY; MICROCHEMISTRY; RATS; GANGLIA, BASAL; NERVE TISSUE PROTEINS; PINEAL BODY; PROTEINS; CEREBRAL CORTEX; HYPOTHALAMUS
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/0003-2697(64)90079-X

A polyacrylamide gel microelectrophoresis apparatus and techniques to fractionate cytoplasmic proteins contained in supernatant from 0.8 to 2 mg rat brain tissue are described. This system provides a marked increase in resolution over previously described methods. It allows the use of minimal amount of proteins in manageable sample sizes. It has the advantage of rapid application of stain, fixative, or precipitable substrate without removing the gel from its protective container. The relatively great surface area of the gel permits the application of sufficient current to maintain excellent resolution without noticeable heating. This method was applied to the extracts of rat pineal, anterior hypothalamus, putamen, and cortical areas 1, 2, and 3 gray and white matter. As many as 19 bands were observed migrating toward the anode. Major quantitative and minor qualitative variations are found to exist among areas studied.