Measurement of green fluorescence protein in plants: quantification, correlation to expression, rapid screening and differential gene expression

• Published in: Plant science (Limerick) Vol. 142; no. 1; pp. 93 - 99
• Main Authors: Blumenthal, A., Kuznetzova, L., Edelbaum, O., Raskin, V., Levy, M., Sela, I.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 02.03.1999; Elsevier Science
• Subjects: Biological and medical sciences; CONTROLE CONTINU; EXPRESION GENICA; EXPRESSION DES GENES; FLUORESCENCE; FLUORESCENCIA; Fundamental and applied biological sciences. Psychology; GENE EXPRESSION; Gene expression monitoring and quantification; GENETIC MARKERS; Green fluorescence protein; MARCADORES GENETICOS; MARQUEUR GENETIQUE; MEASUREMENT; MEDICION; MESURE; Molecular and cellular biology; Molecular genetics; MONITORING; NICOTIANA TABACUM; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; Plants; TRANSGENIC PLANTS; VIGILANCIA; Gene expression monitoring and quantification; Green fluorescence protein; Plants; Dicotyledones; Angiospermae; Green fluorescent protein; Fluorescence; Spermatophyta; Transgenic plant; Solanaceae; Experimental plant; Gene expression; Nicotiana tabacum; Quantitative analysis
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/S0168-9452(98)00249-0

A set-up for detection of green fluorescence protein (GFP), enabling rapid screening of transgenic tobacco is described. Fluorescence levels were proportional to expression levels, and expressing plants were visually selected from a transgenic population. Quantification of GFP expression indicated that the 35S CaMV promoter drives expression of this reporter gene to different levels in different tissues, corroborating previous similar observation with GUS, indicating that constitutive expression varies quantitatively in different tissues.