Measurement of green fluorescence protein in plants: quantification, correlation to expression, rapid screening and differential gene expression

A set-up for detection of green fluorescence protein (GFP), enabling rapid screening of transgenic tobacco is described. Fluorescence levels were proportional to expression levels, and expressing plants were visually selected from a transgenic population. Quantification of GFP expression indicated t...

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Published inPlant science (Limerick) Vol. 142; no. 1; pp. 93 - 99
Main Authors Blumenthal, A., Kuznetzova, L., Edelbaum, O., Raskin, V., Levy, M., Sela, I.
Format Journal Article
LanguageEnglish
Published Shannon Elsevier Ireland Ltd 02.03.1999
Elsevier Science
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Summary:A set-up for detection of green fluorescence protein (GFP), enabling rapid screening of transgenic tobacco is described. Fluorescence levels were proportional to expression levels, and expressing plants were visually selected from a transgenic population. Quantification of GFP expression indicated that the 35S CaMV promoter drives expression of this reporter gene to different levels in different tissues, corroborating previous similar observation with GUS, indicating that constitutive expression varies quantitatively in different tissues.
Bibliography:1999003830
F30
ISSN:0168-9452
1873-2259
DOI:10.1016/S0168-9452(98)00249-0