Dynamics of end grafted DNA molecules and possible biosensor applications
Polymers that are terminally attached to solid supports, so called brushes, form the basis for a wide variety of different applications in colloidal and biophysical sciences. For grafted charged chains a conducting surface allows to manipulate the brush structure by applying electric fields across t...
Saved in:
Published in | Physica status solidi. A, Applications and materials science Vol. 203; no. 14; pp. 3476 - 3491 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin
WILEY-VCH Verlag
01.11.2006
WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Polymers that are terminally attached to solid supports, so called brushes, form the basis for a wide variety of different applications in colloidal and biophysical sciences. For grafted charged chains a conducting surface allows to manipulate the brush structure by applying electric fields across the brush. The dynamics of oligomeric DNA molecules under the action of repulsive and attractive surface electric fields is studied by Brownian dynamics simulations including hydrodynamic effects and compared to experimental results. The difference in flexibility between double and single stranded DNA molecules leads to a change in the switching dynamics when repeatedly reversing the surface charge. This effect allows to detect hybridization of surface anchored DNA. Similar kinetic changes occur when other molecules bind terminally to DNA, opening the possibility to use end grafted polymers for general biosensing applications. We in particular discuss the influence of the adsorbate size and change on the switching dynamics. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
---|---|
Bibliography: | ArticleID:PSSA200622444 BMBF - No. 03N8713 ark:/67375/WNG-7ZPS0B3F-P istex:4F4E7FEC4BB59AAD0243BABA71F98BC2B72758F6 Fujitsu Laboratories of Europe DFG (via SFB 563 and SPP 1164 Nano- and Microfluidics) |
ISSN: | 1862-6300 1862-6319 |
DOI: | 10.1002/pssa.200622444 |