Surface charge of resident, elicited, and activated mouse peritoneal macrophages

The surface charpa of resident, thioglycollate‐elicited, and Trypanosoma cruzi‐ectivated mouse peritoneal macrophages was analyzed using cell electrophoresis. AN macrophages had a net negative surface charge. Activated macrophages had a lower zeta potential and a higher isoelectrophoretic point than...

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Bibliographic Details
Published inJournal of leukocyte biology Vol. 41; no. 2; pp. 143 - 149
Main Authors Silva Riho, Fernando C., Santos, Ana B.S., Carvalho, Técia M.U., Souza, Wanderley
Format Journal Article
LanguageEnglish
Published United States Society for Leukocyte Biology 01.02.1987
Subjects
Animals
Calcium - pharmacology
cell electrophoresis
Hydrogen-Ion Concentration
Key words
Macrophage Activation
Macrophages - physiology
Mice
neuraminidase
Neuraminidase - physiology
Peritoneal Cavity - cytology
sialic acid
Sialic Acids - physiology
Surface Properties
Thioglycolates - pharmacology
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Summary:The surface charpa of resident, thioglycollate‐elicited, and Trypanosoma cruzi‐ectivated mouse peritoneal macrophages was analyzed using cell electrophoresis. AN macrophages had a net negative surface charge. Activated macrophages had a lower zeta potential and a higher isoelectrophoretic point than resident and elicited macrophages. The populations of resident, elicited, and activated macrophages were heterogeneous in terms of surface charge. The analysis of the effect of the pH of the solution in which the macrophages were suspended on their cellular electrophoretic mobility (EPM) indicated that their surface contained both positively and negatively charged dissociating groups. The contribution of sialic acid residuos to the surface change was determined by analyzing the effect of neuraminidase treatment on the EPM of the cells. Activated mecrophages possessed more sialic add residuos exposed on their surface, and sensitive to the neuraminidase from Clostridium perfrigens, than resident and elicited macrophages. Treatment of the cells with the neuraminidase from Vibrio cholerae, however, reduced the surface charge of all macrophages in about the same extent. Macrophages had their mean EPM reduced when Incubated in the presence of Ca++, suggesting that some cell surface anionogenic sites have Ca++‐binding capacity.
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ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.41.2.143