Detection of Uric Acid in the Presence of Dopamine and High Concentration of Ascorbic Acid Using PDDA Modified Graphite Electrode

• Published in: Electroanalysis (New York, N.Y.) Vol. 21; no. 20; pp. 2198 - 2206
• Main Authors: Manjunatha, Hanumanthrayappa, Nagaraju, Doddahalli Hanumantharayudu, Suresh, Gurukar Shivappa, Venkatesha, Thimmappa Venkatarangaiah
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.10.2009; WILEY‐VCH Verlag
• Subjects: Ascorbic acid; Dopamine; Graphite; Medicinal chemistry; Poly(diallyl dimethyl ammonium chloride); Uric acid
• ISSN: 1040-0397; 1521-4109
• DOI: 10.1002/elan.200904662

The properties of graphite electrode (Gr) modified with poly(diallyl dimethyl ammonium chloride) (PDDA) for the detection of uric acid (UA) in the presence of dopamine (DA) and high concentration of ascorbic acid (AA) have been investigated by cyclic voltammetry, differential pulse voltammetry and chronoamperometry. The polymer modified graphite electrode was prepared by a very simple method just by immersing the graphite electrode in PDDA solution for 20 minutes. The PDDA/Gr modified electrode displayed excellent electrocatalytic activity towards the oxidation of UA, DA and AA compared to that at the bare graphite electrode. The electrochemical oxidation signals of UA, DA and AA are well resolved into three distinct peaks with peak potential separations of 220 mV, 168 mV and 387 mV between AA‐DA, DA‐UA and AA‐UA respectively in cyclic voltammetry studies and the corresponding peak potential separations are 230 mV, 130 mV and 354 mV respectively in differential pulse voltammetry. The lowest detection limits obtained for UA, DA and AA were 1×10−7 M, 2×10−7 M and 800×10−9 M respectively. The PDDA/Gr electrode efficiently eliminated the interference of DA and a high concentration of AA in the determination of UA with good selectivity, sensitivity and reproducibility. The modified electrode was also successfully applied for simultaneous determination of UA, DA and AA in their ternary mixture.