Electrochemical Nanobiosensor for Glyphosate Herbicide and Its Metabolite
The use of amperometric biosensor for the detection of glyphosate herbicide and its metabolite aminomethylphosphonic acid (AMPA) is presented. The biosensor was developed by electrochemically depositing poly(2,5‐dimethoxyaniline) (PDMA) doped with poly(4‐styrenesulfonic acid) (PSS) onto the surface...
Saved in:
Published in | Electroanalysis (New York, N.Y.) Vol. 21; no. 3-5; pp. 671 - 674 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
01.02.2009
WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The use of amperometric biosensor for the detection of glyphosate herbicide and its metabolite aminomethylphosphonic acid (AMPA) is presented. The biosensor was developed by electrochemically depositing poly(2,5‐dimethoxyaniline) (PDMA) doped with poly(4‐styrenesulfonic acid) (PSS) onto the surface of a gold electrode followed by electrostatic attachment of horseradish peroxidase (HRP) onto the PDMA‐PSS nanocomposite film. The PDMA‐PSS film was characterized by transmission electron microscopy (TEM) and Fourier Transform infrared (FTIR) spectroscopy. The HRP immobilized on the PDMA‐PSS film catalyzed the reduction of hydrogen peroxide, the inhibition of which was applied in the detection of glyphosate and AMPA. The limits of detection of the biosensor for glyphosate and AMPA were 0.16 μg L−1 and 1.0 μg L−1, respectively. The study demonstrates that the biosensor is very sensitive and could be a useful tool in the screening of glyphosate and AMPA at low concentrations. |
---|---|
Bibliography: | ark:/67375/WNG-1BB81RTQ-F ArticleID:ELAN200804452 istex:E2B9CDC394E5B41E8E5333E0237EE00C1643D327 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 1040-0397 1521-4109 |
DOI: | 10.1002/elan.200804452 |