Identification of extrachromosomal DNA in hematolymphoid cells of chickens and mice

Extrachromosomal DNA of heterogeneous size has been isolated from bursal lymphocytes and splenocytes of five week old chickens, and from splenocytes of mice. This DNA contains covalently closed circles, open circles, and open circles with tails. Open circular molecules with and without tails are mor...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 105; no. 3; pp. 977 - 984
Main Authors Bertelsen, Arthur H., Humayun, M.Zafri, Lippman, Andrea, Rush, Mark G.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 14.04.1982
Subjects
Animals
Chickens
Chromosomes - analysis
DNA, Circular - isolation & purification
Lymphocytes - analysis
Mice
Microscopy, Electron
Molecular Weight
Spleen - analysis
sigma shaped circular DNA
Form I
Form Il
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Summary:Extrachromosomal DNA of heterogeneous size has been isolated from bursal lymphocytes and splenocytes of five week old chickens, and from splenocytes of mice. This DNA contains covalently closed circles, open circles, and open circles with tails. Open circular molecules with and without tails are more frequent than covalently closed species, and the total number of small circular DNA molecules per cell is in the range of 100–200 copies.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(82)91066-X