Measurement of intracellular magnesium in a vascular smooth muscle cell line using a fluorescent probe

Until recently, direct measurement of intracellular free magnesium has been complex and difficult. However, fluorescent probes are now available, based on the same principle as well-established probes for free calcium. Using one such probe, mag-fura-2, we have estimated basal intracellular magnesium...

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Published inBiochimica et biophysica acta Vol. 1035; no. 3; pp. 378 - 380
Main Authors Schachter, M., Gallagher, K.L., Sever, P.S.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 14.09.1990
Subjects
Animals
Calcium
Calcium - metabolism
Calcium - pharmacology
Cell Line
Fluorescent probe
Fura-2
Kinetics
Magnesium
Magnesium - pharmacology
Muscle, Smooth, Vascular - drug effects
Muscle, Smooth, Vascular - metabolism
Rats
Vascular smooth muscle
Calcium
Fluorescent probe
Hepes
[Mg] i
BAPTA/AM
Magnesium
Vascular smooth muscle
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Summary:Until recently, direct measurement of intracellular free magnesium has been complex and difficult. However, fluorescent probes are now available, based on the same principle as well-established probes for free calcium. Using one such probe, mag-fura-2, we have estimated basal intracellular magnesium concentrations in the A7r5 rat vascular smooth muscle cell line. This level unaffected by numerous pharmacological manipulations, including agonist stimulation and depolarisation. The possible implications of these findings are discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(90)90103-4