Synthesis of ppGpp by mouse embryonic ribosomes

The unusual nucleotide guanosine tetraphosphate (ppGpp) is synthesized in vitro by ribosomes isolated from mouse embryos, but is not formed by ribosomes isolated from adults. Analysis of specific organs shows a developmental change in detectable ppGpp-forming ability. Eleven day embryonic liver ribo...

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Bibliographic Details
Published inCell Vol. 3; no. 3; pp. 249 - 253
Main Authors Irr, J.D., Kaulenas, M.S., Unsworth, B.R.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.11.1974
Subjects
Animals
Brain - metabolism
Cell Differentiation
Chromatography, Thin Layer
Escherichia coli - analysis
Guanine Nucleotides - biosynthesis
Kidney - analysis
Liver - embryology
Liver - metabolism
Mice
Protein Biosynthesis
Ribosomes - analysis
Ribosomes - embryology
Ribosomes - metabolism
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Summary:The unusual nucleotide guanosine tetraphosphate (ppGpp) is synthesized in vitro by ribosomes isolated from mouse embryos, but is not formed by ribosomes isolated from adults. Analysis of specific organs shows a developmental change in detectable ppGpp-forming ability. Eleven day embryonic liver ribosomes synthesize ppGpp, but this ability is essentially lost by 14 day embryonic liver and adult liver. Eleven day embryonic brain ribosomes also synthesize ppGpp at a level comparable to that observed using E. coli ribosomes. The synthesis of ppGpp appears to be associated with the early stages of differentiation, when cell proliferation is rapid and specialized protein synthesis is low or absent. The potential role of ppGpp as an effector molecule or regulator in the eucaryotes is discussed.
Bibliography:ObjectType-Article-1
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ISSN:0092-8674
1097-4172
DOI:10.1016/0092-8674(74)90139-1