Glyco-gold nanoparticle shapes enhance carbohydrate-protein interactions in mammalian cells

Advances in shape-dependent nanoparticle (NP) research have prompted a close scrutiny of the behaviour of nanostructures in vitro and in vivo. Data pertaining to cellular uptake and site specific sequestration of different shapes of NPs will undoubtedly assist researchers to design better nano-probe...

Full description

Saved in:
Bibliographic Details
Published inNanoscale Vol. 8; no. 25; pp. 12729 - 12735
Main Authors Sangabathuni, Sivakoti, Vasudeva Murthy, Raghavendra, Chaudhary, Preeti Madhukar, Surve, Manalee, Banerjee, Anirban, Kikkeri, Raghavendra
Format Journal Article
LanguageEnglish
Published England 07.07.2016
Subjects
Animals
Biotechnology
Carbohydrates
Endocytosis
Galactose - chemistry
Gold
HeLa Cells
Humans
Lectins - chemistry
Mammals
Mannose
Mannose - chemistry
Metal Nanoparticles
Mice
Nanoparticles
Nanostructure
NIH 3T3 Cells
Proteins - chemistry
Stars
Uptakes
Online AccessGet full text

Cover

More Information
Summary:Advances in shape-dependent nanoparticle (NP) research have prompted a close scrutiny of the behaviour of nanostructures in vitro and in vivo. Data pertaining to cellular uptake and site specific sequestration of different shapes of NPs will undoubtedly assist researchers to design better nano-probes for therapeutic and imaging purposes. Herein, we investigated the shape dependent uptake of glyco-gold nanoparticles (G-AuNPs) in different cancer cell lines. Specifically, we have compared the behaviour of spherical, rod and star AuNPs with mannose and galactose conjugations. In vitro experiments showed that the rod-AuNPs exhibited the highest uptake over that of the star and spherical counterparts. Further, an investigation of the mechanism of the uptake clearly demonstrated clathrin mediated endocytosis of the specific G-AuNPs. These results reveal the benefits of different G-AuNP shapes in carbohydrate-mediated interactions.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2040-3364
2040-3372
DOI:10.1039/c6nr03008d