Freeze-thaw behavior of a moderately halophilic bacterium as a function of salt concentration
A moderately halophilic bacterium was studied with respect to growth and survival after freezing and thawing at various cooling and warming rates and in various sodium chloride concentrations. After freezing and thawing log phase cultures, surviving cells continued in log growth. A portion of the fr...
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Published in | Cryobiology Vol. 7; no. 2; pp. 107 - 112 |
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Main Author | |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier Inc
01.09.1970
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Subjects | |
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Abstract | A moderately halophilic bacterium was studied with respect to growth and survival after freezing and thawing at various cooling and warming rates and in various sodium chloride concentrations. After freezing and thawing log phase cultures, surviving cells continued in log growth. A portion of the freeze-thaw-induced cell damage could be reversed by incubating in the medium in which the cells were grown. Viability was dependent on cooling and warming rates and salt concentration. The combination of 20 °C per min cooling, 400 °C per min warming (fastest warming rate used), and 10% salt yielded the highest survival. |
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AbstractList | A moderately halophilic bacterium was studied with respect to growth and survival after freezing and thawing at various cooling and warming rates and in various sodium chloride concentrations. After freezing and thawing log phase cultures, surviving cells continued in log growth. A portion of the freeze-thaw-induced cell damage could be reversed by incubating in the medium in which the cells were grown. Viability was dependent on cooling and warming rates and salt concentration. The combination of 20 °C per min cooling, 400 °C per min warming (fastest warming rate used), and 10% salt yielded the highest survival. |
Author | Deal, Paul H. |
Author_xml | – sequence: 1 givenname: Paul H. surname: Deal fullname: Deal, Paul H. organization: Exobiology Division, Ames Research Center, National Aeronautics and Space Administration, Moffett Field, California U.S.A |
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Cites_doi | 10.1126/science.158.3797.78 10.1111/j.1365-2672.1963.tb04791.x 10.1128/JB.89.3.718-724.1965 10.1128/JB.85.2.485-485.1963 10.1016/S0011-2240(69)80007-6 10.1128/JB.78.2.181-185.1959 10.1038/1991078a0 10.1007/BF00924233 10.1128/AEM.3.1.40-45.1955 10.1016/S0011-2240(66)80165-7 10.1002/jobm.3630040202 10.1007/BF02868761 10.1016/S0011-2240(68)80138-5 10.1016/S0011-2240(68)80111-7 10.1128/AEM.10.4.297-301.1962 |
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References | Young, Deal, Whitfield (BIB17) 1968; 1 Doebbler, Rinfret (BIB5) 1963; 85 Mazur, Schmidt (BIB9) 1968; 5 Young, Deal, Bell, Allen (BIB16) 1964 Levy (BIB6) 1969; 6 Mazur (BIB8) 1966; 2 Postgate, Hunter (BIB11) 1963; 26 Arpai (BIB1) 1962; 10 Arpai (BIB3) 1964; 4 Bowman, Blume, Vurek (BIB4) 1967; 158 Stapert, Sokolski (BIB13) 1968; 4 Straka, Stokes (BIB14) 1959; 78 Mazur (BIB7) 1966 Packer, Ingraham, Scher (BIB10) 1965; 89 Arpai (BIB2) 1963; 8 Young, Deal, Bell, Allen (BIB15) 1963; 199 Squires, Hartsell (BIB12) 1955; 3 Stapert (10.1016/0011-2240(70)90005-2_BIB13) 1968; 4 Young (10.1016/0011-2240(70)90005-2_BIB17) 1968; 1 Squires (10.1016/0011-2240(70)90005-2_BIB12) 1955; 3 Arpai (10.1016/0011-2240(70)90005-2_BIB1) 1962; 10 Levy (10.1016/0011-2240(70)90005-2_BIB6) 1969; 6 Mazur (10.1016/0011-2240(70)90005-2_BIB7) 1966 Mazur (10.1016/0011-2240(70)90005-2_BIB8) 1966; 2 Arpai (10.1016/0011-2240(70)90005-2_BIB3) 1964; 4 Straka (10.1016/0011-2240(70)90005-2_BIB14) 1959; 78 Arpai (10.1016/0011-2240(70)90005-2_BIB2) 1963; 8 Bowman (10.1016/0011-2240(70)90005-2_BIB4) 1967; 158 Doebbler (10.1016/0011-2240(70)90005-2_BIB5) 1963; 85 Packer (10.1016/0011-2240(70)90005-2_BIB10) 1965; 89 Mazur (10.1016/0011-2240(70)90005-2_BIB9) 1968; 5 Postgate (10.1016/0011-2240(70)90005-2_BIB11) 1963; 26 Young (10.1016/0011-2240(70)90005-2_BIB15) 1963; 199 Young (10.1016/0011-2240(70)90005-2_BIB16) 1964 |
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Bact contributor: fullname: Scher – volume: 158 start-page: 78 year: 1967 end-page: 83 ident: BIB4 article-title: Capillary-tube scanner for mechanized microbiology publication-title: Science contributor: fullname: Vurek – volume: 2 start-page: 181 year: 1966 end-page: 192 ident: BIB8 article-title: Theoretical and experimental effects of cooling and warming velocity on the survival of frozen and thawed cells publication-title: Cryobiology contributor: fullname: Mazur – volume: 4 start-page: 105 year: 1964 end-page: 113 ident: BIB3 article-title: On the recovery of bacteria from freezing publication-title: Z. Alleg. Mikrobiol contributor: fullname: Arpai – volume: 1 start-page: 113 year: 1968 end-page: 117 ident: BIB17 article-title: The response of spore-forming vs. nonspore-forming bacteria to diurnal freezing and thawing publication-title: Space Life Sci contributor: fullname: Whitfield – volume: 26 start-page: 405 year: 1963 end-page: 414 ident: BIB11 article-title: Metabolic injury in frozen bacteria publication-title: J. Appl. Bact contributor: fullname: Hunter – start-page: 105 year: 1964 end-page: 111 ident: BIB16 article-title: Bacteria under simulated Martian conditions publication-title: Life Sciences and Space Research II contributor: fullname: Allen – volume: 199 start-page: 1078 year: 1963 end-page: 1079 ident: BIB15 article-title: Effect of diurnal freeze-thawing on survival and growth of selected bacteria publication-title: Nature contributor: fullname: Allen – volume: 78 start-page: 181 year: 1959 end-page: 185 ident: BIB14 article-title: Metabolic injury to bacteria at low temperatures publication-title: J. Bact contributor: fullname: Stokes – volume: 10 start-page: 297 year: 1962 end-page: 301 ident: BIB1 article-title: Nonlethal freezing injury to metabolism and motility of publication-title: Appl. 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Bact doi: 10.1128/JB.78.2.181-185.1959 contributor: fullname: Straka – start-page: 213 year: 1966 ident: 10.1016/0011-2240(70)90005-2_BIB7 article-title: Physical and chemical basis of injury in single celled microorganisms subjected to freezing and thawing contributor: fullname: Mazur – volume: 199 start-page: 1078 year: 1963 ident: 10.1016/0011-2240(70)90005-2_BIB15 article-title: Effect of diurnal freeze-thawing on survival and growth of selected bacteria publication-title: Nature doi: 10.1038/1991078a0 contributor: fullname: Young – volume: 1 start-page: 113 year: 1968 ident: 10.1016/0011-2240(70)90005-2_BIB17 article-title: The response of spore-forming vs. nonspore-forming bacteria to diurnal freezing and thawing publication-title: Space Life Sci doi: 10.1007/BF00924233 contributor: fullname: Young – volume: 3 start-page: 40 year: 1955 ident: 10.1016/0011-2240(70)90005-2_BIB12 article-title: Survival and growth initiation of defrosted Escherichia coli as affected by frozen storage menstrua publication-title: Appl. 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Mikrobiol doi: 10.1002/jobm.3630040202 contributor: fullname: Arpai – start-page: 105 year: 1964 ident: 10.1016/0011-2240(70)90005-2_BIB16 article-title: Bacteria under simulated Martian conditions contributor: fullname: Young – volume: 8 start-page: 18 year: 1963 ident: 10.1016/0011-2240(70)90005-2_BIB2 article-title: Selective effect of freezing as reflected in growth curves publication-title: Folia Microbiol doi: 10.1007/BF02868761 contributor: fullname: Arpai – volume: 5 start-page: 1 year: 1968 ident: 10.1016/0011-2240(70)90005-2_BIB9 article-title: Interactions of cooling velocity, temperature, and warming velocity on the survival of frozen and thawed yeast publication-title: Cryobiology doi: 10.1016/S0011-2240(68)80138-5 contributor: fullname: Mazur – volume: 4 start-page: 174 year: 1968 ident: 10.1016/0011-2240(70)90005-2_BIB13 article-title: The effect of freezing and thawing on cellular function in Lactobacillus leichmannii publication-title: Cryobiology doi: 10.1016/S0011-2240(68)80111-7 contributor: fullname: Stapert – volume: 10 start-page: 297 year: 1962 ident: 10.1016/0011-2240(70)90005-2_BIB1 article-title: Nonlethal freezing injury to metabolism and motility of Pseudomonas fluorescens and Escherichia coli publication-title: Appl. Microbiol doi: 10.1128/AEM.10.4.297-301.1962 contributor: fullname: Arpai |
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SubjectTerms | Bacteria - drug effects Bacteria - growth & development |
Title | Freeze-thaw behavior of a moderately halophilic bacterium as a function of salt concentration |
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