Freeze-thaw behavior of a moderately halophilic bacterium as a function of salt concentration

A moderately halophilic bacterium was studied with respect to growth and survival after freezing and thawing at various cooling and warming rates and in various sodium chloride concentrations. After freezing and thawing log phase cultures, surviving cells continued in log growth. A portion of the fr...

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Published inCryobiology Vol. 7; no. 2; pp. 107 - 112
Main Author Deal, Paul H.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Inc 01.09.1970
Subjects
Bacteria - drug effects
Bacteria - growth & development
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Abstract A moderately halophilic bacterium was studied with respect to growth and survival after freezing and thawing at various cooling and warming rates and in various sodium chloride concentrations. After freezing and thawing log phase cultures, surviving cells continued in log growth. A portion of the freeze-thaw-induced cell damage could be reversed by incubating in the medium in which the cells were grown. Viability was dependent on cooling and warming rates and salt concentration. The combination of 20 °C per min cooling, 400 °C per min warming (fastest warming rate used), and 10% salt yielded the highest survival.
AbstractList A moderately halophilic bacterium was studied with respect to growth and survival after freezing and thawing at various cooling and warming rates and in various sodium chloride concentrations. After freezing and thawing log phase cultures, surviving cells continued in log growth. A portion of the freeze-thaw-induced cell damage could be reversed by incubating in the medium in which the cells were grown. Viability was dependent on cooling and warming rates and salt concentration. The combination of 20 °C per min cooling, 400 °C per min warming (fastest warming rate used), and 10% salt yielded the highest survival.
Author Deal, Paul H.
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CitedBy_id crossref_primary_10_1016_0011_2240_74_90033_9
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crossref_primary_10_1080_09593331003782417
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Cites_doi 10.1126/science.158.3797.78
10.1111/j.1365-2672.1963.tb04791.x
10.1128/JB.89.3.718-724.1965
10.1128/JB.85.2.485-485.1963
10.1016/S0011-2240(69)80007-6
10.1128/JB.78.2.181-185.1959
10.1038/1991078a0
10.1007/BF00924233
10.1128/AEM.3.1.40-45.1955
10.1016/S0011-2240(66)80165-7
10.1002/jobm.3630040202
10.1007/BF02868761
10.1016/S0011-2240(68)80138-5
10.1016/S0011-2240(68)80111-7
10.1128/AEM.10.4.297-301.1962
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Doebbler, Rinfret (BIB5) 1963; 85
Mazur, Schmidt (BIB9) 1968; 5
Young, Deal, Bell, Allen (BIB16) 1964
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Stapert (10.1016/0011-2240(70)90005-2_BIB13) 1968; 4
Young (10.1016/0011-2240(70)90005-2_BIB17) 1968; 1
Squires (10.1016/0011-2240(70)90005-2_BIB12) 1955; 3
Arpai (10.1016/0011-2240(70)90005-2_BIB1) 1962; 10
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Mazur (10.1016/0011-2240(70)90005-2_BIB7) 1966
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Arpai (10.1016/0011-2240(70)90005-2_BIB3) 1964; 4
Straka (10.1016/0011-2240(70)90005-2_BIB14) 1959; 78
Arpai (10.1016/0011-2240(70)90005-2_BIB2) 1963; 8
Bowman (10.1016/0011-2240(70)90005-2_BIB4) 1967; 158
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Packer (10.1016/0011-2240(70)90005-2_BIB10) 1965; 89
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Young (10.1016/0011-2240(70)90005-2_BIB15) 1963; 199
Young (10.1016/0011-2240(70)90005-2_BIB16) 1964
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Snippet A moderately halophilic bacterium was studied with respect to growth and survival after freezing and thawing at various cooling and warming rates and in...
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SubjectTerms Bacteria - drug effects
Bacteria - growth & development
Title Freeze-thaw behavior of a moderately halophilic bacterium as a function of salt concentration
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