Freeze-thaw behavior of a moderately halophilic bacterium as a function of salt concentration

A moderately halophilic bacterium was studied with respect to growth and survival after freezing and thawing at various cooling and warming rates and in various sodium chloride concentrations. After freezing and thawing log phase cultures, surviving cells continued in log growth. A portion of the fr...

Full description

Saved in:
Bibliographic Details
Published inCryobiology Vol. 7; no. 2; pp. 107 - 112
Main Author Deal, Paul H.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Inc 01.09.1970
Subjects
Bacteria - drug effects
Bacteria - growth & development
Online AccessGet full text

Cover

More Information
Summary:A moderately halophilic bacterium was studied with respect to growth and survival after freezing and thawing at various cooling and warming rates and in various sodium chloride concentrations. After freezing and thawing log phase cultures, surviving cells continued in log growth. A portion of the freeze-thaw-induced cell damage could be reversed by incubating in the medium in which the cells were grown. Viability was dependent on cooling and warming rates and salt concentration. The combination of 20 °C per min cooling, 400 °C per min warming (fastest warming rate used), and 10% salt yielded the highest survival.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0011-2240
1090-2392
DOI:10.1016/0011-2240(70)90005-2