Quantitation of chemotactic factor generation in human sera

• Published in: Journal of immunological methods Vol. 36; no. 3-4; p. 349
• Main Authors: Tannous, R, Cavender, N
• Format: Journal Article
• Language: English
• Published: Netherlands 01.01.1980
• Subjects: Adult; Chemotactic Factors - biosynthesis; Chemotaxis, Leukocyte - drug effects; Female; Gels; Humans; Male; Neutrophils - physiology; Polysaccharides; Sepharose; Serum Albumin, Bovine; Zymosan - pharmacology

Increased sensitivity of chemotaxin titration in the 'migration under agarose' method was achieved by reduction in agarose concentration of the medium from the conventional 1.0% to 0.5%. Satisfactory technical properties of the gel were achieved by inclusion of 2.0% bovine serum albumin. T...