Quantitation of chemotactic factor generation in human sera

Increased sensitivity of chemotaxin titration in the 'migration under agarose' method was achieved by reduction in agarose concentration of the medium from the conventional 1.0% to 0.5%. Satisfactory technical properties of the gel were achieved by inclusion of 2.0% bovine serum albumin. T...

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Bibliographic Details
Published inJournal of immunological methods Vol. 36; no. 3-4; p. 349
Main Authors Tannous, R, Cavender, N
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1980
Subjects
Adult
Chemotactic Factors - biosynthesis
Chemotaxis, Leukocyte - drug effects
Female
Gels
Humans
Male
Neutrophils - physiology
Polysaccharides
Sepharose
Serum Albumin, Bovine
Zymosan - pharmacology
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Summary:Increased sensitivity of chemotaxin titration in the 'migration under agarose' method was achieved by reduction in agarose concentration of the medium from the conventional 1.0% to 0.5%. Satisfactory technical properties of the gel were achieved by inclusion of 2.0% bovine serum albumin. The modificatin resulted in increased migration rates and greater reproducibility than those achieved with other media. The range of normal values was established by study of sera of 20 normal adults with use of a single batch of target cells. It is concluded that under the conditions described, a difference in chemotactic differential of 10% or more between a serum sample submitted for assay and that of pooled serum from normal subjects is statistically significant.
ISSN:0022-1759
DOI:10.1016/0022-1759(80)90140-4