Changes in culture medium pH by cell suspension cultures of Dioscorea deltoidea

• Published in: Plant science letters Vol. 35; no. 3; pp. 207 - 212
• Main Authors: Butenko, R.G., Lipsky, A.Kh, Chernyak, N.D., Arya, H.C.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.01.1984; Elsevier
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Biotechnology; cell suspension cultures; changes in pH; Dioscorea; Economic plant physiology; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; growth; In vitro culture; In vitro propagation: entire plant regeneration from tissues and cell cultures; Methods. Procedures. Technologies; Plant cells and fungal cells; Plant physiology and development; respiration; Tissue cultures, protoplasts; yams; MS; cell suspension cultures; pH i initial pH of culture medium; changes in pH; Dioscorea; pH c; growth; respiration; 2,4-D, 2-4-dichlorophenoxyacetic acid; Culture medium; Cell culture; Monocotyledones; Suspension culture; Angiospermae; Tissue culture; PH; Spermatophyta; Respiration; Dioscoreaceae
• ISSN: 0304-4211
• DOI: 10.1016/0304-4211(84)90230-X

The effect of initial pH of the culture medium (pH i ) on growth, changes in pH of culture medium (pH c ) and respiration of Dioscorea deltoidea cells was studied in cycle of batch cultivation. At pH i 3.50 the lag phase of growth and respiration was about 24 h. Within the pH i -range of 4.30–6.15 no lag phase was observed, growth and respiration being similar. At pH i -values of 2.00–2.50 and 8.00 the cell population died. At pH 3.50–6.15 the level of pH c was shifted to 4.6–4.7 by cells within 10–48 h. We suppose that the pH-regulation at pH i range of 3.50–6.15 by the cells to a definite value of the pH c (4.6–4.7) ensures their normal growthy cycle.