Separation of immunologically active fragments by membrane partition chromatography

A system has been described for the separation of the partially degraded products of an immunoreactive preparation using acrylamide-entrapped enzyme, and a tandem series of ultrafiltration cells, each equipped with a different molecular size retentive filter. This technique of digestion with concomi...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 30; no. 1; pp. 91 - 98
Main Authors Zipilivan, E.M., Hudson, B.G., Blatt, W.F.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.07.1969
Subjects
Animals
Antigens - isolation & purification
Caseins
Chromatography, Gel
Chymotrypsin
Filtration
Immunodiffusion
Immunoelectrophoresis
Membranes, Artificial
Methods
Rabbits
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Summary:A system has been described for the separation of the partially degraded products of an immunoreactive preparation using acrylamide-entrapped enzyme, and a tandem series of ultrafiltration cells, each equipped with a different molecular size retentive filter. This technique of digestion with concomitant ultrafiltration affords a means of readily obtaining immunologically reactive fragments of intermediate size. Verification of the changes in antigenicity was obtained by immunodiffusion and immunoelectrophoresis of the membrane-separated components of the enzyme-degraded mixture.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(69)90376-5