Isolation of phenanthrene-degrading bacteria and characterization of phenanthrene metabolites

• Published in: World journal of microbiology & biotechnology Vol. 23; no. 5; pp. 647 - 654
• Main Authors: Tao, Xue-Qin, Lu, Gui-Ning, Dang, Zhi, Yi, Xiao-Yun, Yang, Chen
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Kluwer Academic Publishers 01.05.2007; Springer; Springer Nature B.V
• Subjects: Aromatic compounds; Aromatic hydrocarbons; Bacteria; Batch culture; Benzene; Biodegradation; Biological and medical sciences; Biotechnology; Carbon; Catechol; Cell culture; Cell number; Consortia; Degradation pathway; Degradation products; Energy sources; Fundamental and applied biological sciences. Psychology; Gas chromatography; Mass spectrometry; Mass spectroscopy; Metabolites; Microbiology; Naphthalene; Naphthol; Oil pollution; Phenanthrene; Phenols; Polycyclic aromatic hydrocarbons; Polycyclic aromatic hydrocarbons (PAHs); Salicylic acid; Salts; Silicones; Soil contamination; Soil pollution; Soil water; Sphingomonas; Sphingomonas sp; Toluene; Characterization; Degradation; Phenanthrene; Hydrocarbon; Metabolite; Bacteria; Isolation; Sphingomonas; Polycyclic aromatic compound; Degradation product; Polycyclic aromatic hydrocarbons (PAHs), Sphingomonas sp; Degradation pathway, Degradation products Phenanthrene
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-006-9276-4

Three aerobic bacterial consortia GY2, GS3 and GM2 were enriched from polycyclic aromatic hydrocarbon-contaminated soils with water-silicone oil biphasic systems. An aerobic bacterial strain utilizing phenanthrene as the sole carbon and energy source was isolated from bacterial consortium GY2 and identified as Sphingomonas sp. strain GY2B. Within 48 h and at 30°C the strain metabolized 99.1% of phenanthrene (100 mg/l) added to batch culture in mineral salts medium and the cell number increased by about 40-fold. Three metabolites 1-hydroxy-2-naphthoic acid, 1-naphthol and salicylic acid, were identified by gas chromatographic mass spectrometry and UV-visible spectroscopy analysis. A degradation pathway was proposed based on the identified metabolites. In addition to phenanthrene, strain GY2B could use other aromatic compounds such as naphthalene, 2-naphthol, salicylic acid, catechol, phenol, benzene and toluene as a sole source of carbon and energy.