GM-CSF is required for the Pseudomonas exotoxin A-induced proliferation of immature T cells in athymic mice

• Published in: Cellular immunology Vol. 160; no. 1; pp. 65 - 70
• Main Authors: Dixon, Diane M., Misfeldt, Michael L.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 1995
• Subjects: ADP Ribose Transferases; Animals; Antibodies, Monoclonal - immunology; Bacterial Toxins; Cell Differentiation - immunology; Exotoxins - immunology; Granulocyte-Macrophage Colony-Stimulating Factor - biosynthesis; Granulocyte-Macrophage Colony-Stimulating Factor - physiology; Interleukin-2 - biosynthesis; Lymphocyte Activation - immunology; Mice; Mice, Nude; Pseudomonas aeruginosa Exotoxin A; Spleen - cytology; T-Lymphocytes - immunology; Thy-1 Antigens - immunology; Tumor Necrosis Factor-alpha - biosynthesis; Virulence Factors
• ISSN: 0008-8749; 1090-2163
• DOI: 10.1016/0008-8749(95)80010-G

Previous studies have demonstrated that Pseudomonas exotoxin A stimulated the proliferation of immature T lymphocytes within the splenocytes of athymic mice. These studies were performed to determine which lymphokines were involved in the proliferation of the immature T cells. The results of this study indicate that exotoxin A does not induce the production of interleukin-2 or tumor necrosis factor from B cell-depleted splenotypes from athymic mice. However, exotoxin A does induce the production of granulocyte-macrophage colony-stimulating factor (GM-CSF) from B cell-depleted splenocytes. Furthermore, the GM-CSF was shown to be produced by a Thy1 +, CD4 −, CD8 − T lymphocyte. The addition of anti-GM-CSF antibody abrogates the exotoxin A-induced proliferation of B cell-depleted splenocytes from athymic mice. Thus, these data indicate that exotoxin A induces the production of GM-CSF from immature T lymphocytes within the splenocytes of athymic mice and the exotoxin A-induced proliferation of these immature T cells is dependent on the presence of GM-CSF.