Overlapping of normal and malignant mouse cells in pure and mixed cultures

Cultured malignant mouse melanoma cells and mouse ascites carcinoma cells, after 24 h incubation with normal mouse cells or with each other, had much higher homologous overlap indices than those recorded from pure cultures of each cell type. The effect was particularly evident in ascites cells. Norm...

Full description

Saved in:
Bibliographic Details
Published inExperimental cell research Vol. 84; no. 1; pp. 95 - 104
Main Authors Stephenson, E.M., Natt, J.K., Stephenson, N.G.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.03.1974
Subjects
Animals
Carcinoma
Cell Count
Cell Division
Cell Line
Cell Movement
Cell Nucleus
Cell Transformation, Neoplastic
Cells, Cultured
Contact Inhibition
Culture Media
Lung
Melanoma
Mice
Mice, Inbred C57BL
Mitosis
Neoplasms, Experimental
Skin
Online AccessGet full text

Cover

More Information
Summary:Cultured malignant mouse melanoma cells and mouse ascites carcinoma cells, after 24 h incubation with normal mouse cells or with each other, had much higher homologous overlap indices than those recorded from pure cultures of each cell type. The effect was particularly evident in ascites cells. Normal cells of mice of different ages showed varying responses in overlap frequency when incubated with malignant cells. Conditioned medium from each malignant cell type invoked increased overlapping of cells of the heterologous malignant type but did not affect homologous cells. The effects of whole conditioned medium on heterologous malignant cells showed a graded reduction after dilution of the medium and also occurred after dialysis. A dialysable chemical factor or factors, produced by the cells, is postulated.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(74)90384-X