Purification of a coagulant protein from seeds of Moringa concanensis

• Published in: Water science & technology. Water supply Vol. 12; no. 3; pp. 329 - 333
• Main Author: SATHIYABAMA, M
• Format: Journal Article
• Language: English
• Published: London International Water Association 01.01.2012; IWA Publishing
• Subjects: Acetates; Acetic acid; Ammonium; Ammonium acetate; Ammonium compounds; Applied sciences; Buffers; Buildings. Public works; Chromatography; Coagulants; Coagulation; Column chromatography; Drinking water; Exact sciences and technology; Incubation period; Molecular weight; Protein purification; Proteins; Seeds; Sodium chloride; Water purification; Water supplies; Water supply. Pipings. Water treatment; Water treatment; Water treatment; Purification; Moringa concanensis; Protein; Coagulant; Dicotyledones; Seed; CM-Sepharose; Angiospermae; coagulant protein; Moringaceae; Spermatophyta; Molecular mass
• ISSN: 1606-9749; 1607-0798
• DOI: 10.2166/ws.2011.140

A coagulant protein from seeds of Moringa concanensis was isolated and purified using CM-Sepharose column chromatography. The column matrix was equilibrated with ammonium acetate buffer and maximum protein was eluted at 0.8 M NaCl. The molecular mass of the purified protein was identified as 14 kDa and its pI value was around 9.5. The purified coagulant protein retained 90% coagulation activity even after incubation at 90 °C for 3 h. The purified protein does not release organic content to the water. This paper suggests that coagulant protein from M. concanenesis can be used in drinking water treatment.