Estimation of nanogram quantities of proteins by pulse-polarographic technique

A pulse-polarographic method of protein estimation has been developed. Bovine serum albumin produces in ammonia buffer in the presence of cobaltic salt one or two waves, respectively, which are linearly proportional to protein concentration in the range of 0.05 to 10 μg/ml. The lowest limit for seru...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 42; no. 1; pp. 59 - 71
Main Authors Paleček, E., Pechan, Z.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.07.1971
Subjects
Amino Acids
Ammonium Chloride
Animals
Cattle
Citrus
Cobalt
Deoxyribonucleases - analysis
Hydroxides
Insulin - analysis
Methods
Microchemistry
Muramidase - analysis
Pancreas - enzymology
Polarography - instrumentation
Proteins - analysis
Quaternary Ammonium Compounds
Ribonucleases - analysis
Saliva - analysis
Serum Albumin, Bovine - analysis
Serum Albumin, Bovine - urine
Spectrophotometry
Surface-Active Agents
Trypsin - analysis
Ultraviolet Rays
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Summary:A pulse-polarographic method of protein estimation has been developed. Bovine serum albumin produces in ammonia buffer in the presence of cobaltic salt one or two waves, respectively, which are linearly proportional to protein concentration in the range of 0.05 to 10 μg/ml. The lowest limit for serum albumin estimation is around 5 nanograms. The method is relatively specific and the determination is little affected by the presence of other substances. An appropriate standard should be used because of the different polarographic activities of various proteins. Practical applicability of the method has been verified by the analysis of some biological materials of animal and plant origin.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(71)90010-8