The relationship between IGF1 and the expression spectrum of miRNA in the placenta of preeclampsia patients

• Published in: Ginekologia polska Vol. 90; no. 10; pp. 596 - 603
• Main Authors: He, Xiaoju, Zhao, Lu, Yue, Lulu, Zhang, Wei, Wang, Wanzhen, Fu, Yuanping, Feng, Yueying, Fu, Fen
• Format: Journal Article
• Language: English
• Published: Poland Wydawnictwo Via Medica 01.01.2019
• Subjects: Cluster Analysis; Computer simulation; Female; Humans; Insulin-Like Growth Factor I - genetics; Insulin-Like Growth Factor I - metabolism; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; Placenta; Placenta - chemistry; Placenta - metabolism; Pre-Eclampsia - epidemiology; Pre-Eclampsia - genetics; Pre-Eclampsia - metabolism; Preeclampsia; Pregnancy; Signal Transduction - genetics; miRNA; placenta; IGF1; preeclampsia; expression spectrum
• ISSN: 0017-0011; 2543-6767
• DOI: 10.5603/GP.2019.0096

Pre-eclampsia (PE) affects many women worldwide and remains the leading cause of morbidity and mortality in neonatal and maternal settings. Abnormal expression of placental microRNAs (miRNAs) may be associated with PE. This study was conducted to the relationship between IGF1 and the expression spectrum of miRNA in the placenta of preeclampsia patient. The expression of miRNA in placental tissue was compared between pre-eclampsia (n = 6) and normal pregnant women (n = 5) miRNA targets were studied by computer simulation and functional assays. The role of miRNA was verified in trophoblast cell lines by apoptosis assay and invasion assay. There was a significant increase in miRNAs in the placenta of women with pre-eclampsia compared with patients with normal pregnancy. Luciferase assay confirmed direct regulation of miRNA. The expression of IGF1 and miRNA was significantly increased in the placenta of patients with pre-eclampsia.