Genomic sequence analysis of a new reassortant infectious bursal disease virus from commercial broiler flocks in central China

• Published in: Archives of virology Vol. 158; no. 9; pp. 1973 - 1978
• Main Authors: Cui, Pei, Ma, Shi-Jie, Zhang, Yu-Geng, Li, Xin-Sheng, Gao, Xiao-Yun, Cui, Bao-An, Chen, Hong-Ying
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.09.2013; Springer Nature B.V
• Subjects: Amino Acid Sequence; Amino acids; Animals; Anorexia; Base Sequence; Biomedical and Life Sciences; Biomedicine; Birnaviridae Infections - veterinary; Birnaviridae Infections - virology; Brief Report; Chickens - virology; China; Eggs; Genome, Viral - genetics; Genomes; Immunization; Infectious bursal disease virus; Infectious bursal disease virus - classification; Infectious bursal disease virus - genetics; Infectious bursal disease virus - immunology; Infectious bursal disease virus - isolation & purification; Infectious bursal disease virus - pathogenicity; Infectious Diseases; Leukemia; Medical Microbiology; Open Reading Frames; Phylogenetics; Phylogeny; Poultry; Poultry Diseases - virology; Reassortant Viruses - genetics; RNA polymerase; RNA, Viral - genetics; Sequence Analysis, DNA; Vaccines; Vaccines, Attenuated - genetics; Vaccines, Attenuated - immunology; Virology; Virulence; Viruses; China; Henan China; United States > US; Japan; Unique Amino Acid; Infectious Bursal Disease Virus; Deduce Amino Acid Sequence; Nucleotide Sequence Identity; Murine Leukemia Virus Reverse Transcriptase
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-013-1682-y

We report the complete nucleotide sequence of a reassortant infectious bursal disease (IBD) virus (IBDV) HN isolate from commercial broiler flocks in central China. The genome consisted of 3,232 and 2,652 nucleotides in the coding regions of segments A and B, respectively. Alignment of both nucleotide and deduced amino acid sequences and phylogenetic analysis revealed that the genome segments A and B of HN were derived from the attenuated strain B87 and the VV strain OKYM. This is a new reassortant IBDV strain that has emerged in nature, involving segment A of a cell-culture-adapted attenuated vaccine strain B87.