An evaluation of some factors important for maximising sensitivity of plant virus detection by immuno-electron microscopy

We report the results of a comparison of the variations to the basic technique developed by Derrick and called ‘serologically specific electron microscopy’ by him. We have found that the reaction time of the antibody-coated grid with the virus preparation (virus acquisition time) is one of the key f...

Full description

Saved in:
Bibliographic Details
Published inJournal of virological methods Vol. 11; no. 4; pp. 339 - 346
Main Authors Pares, R.D., Whitecross, M.I.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.08.1985
Subjects
Immunoassay
Microscopy, Electron
plant virus detection immuno-electron microscopy
Plant Viruses - isolation & purification
Staphylococcal Protein A
Time Factors
plant virus detection immuno-electron microscopy
Online AccessGet full text

Cover

More Information
Summary:We report the results of a comparison of the variations to the basic technique developed by Derrick and called ‘serologically specific electron microscopy’ by him. We have found that the reaction time of the antibody-coated grid with the virus preparation (virus acquisition time) is one of the key factors in determining the efficiency of the method at trapping virus particles. The lower the virus concentration, the longer the reaction time should be. The use of protein A to pretreat the grids before coating with antiserum did not appear to be of benefit under our conditions.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0166-0934
1879-0984
DOI:10.1016/0166-0934(85)90027-8