Reorganisation of the microtubular cytoskeleton by embryonic microtubule-associated protein 2 (MAP2c)

• Published in: Development (Cambridge) Vol. 116; no. 4; pp. 1151 - 1161
• Main Authors: WEISSHAAR, B, DOLL, T, MATUS, A
• Format: Journal Article
• Language: English
• Published: Cambridge The Company of Biologists Limited 01.12.1992; Company of Biologists
• Subjects: Animals; Biological and medical sciences; Calcium-Calmodulin-Dependent Protein Kinases; Cytoskeleton - ultrastructure; Embryology: invertebrates and vertebrates. Teratology; Fundamental and applied biological sciences. Psychology; Microscopy, Electron; Microtubules - ultrastructure; Molecular embryology; Nervous System - embryology; Phenotype; Protein Kinases - physiology; Rats; Transfection; Embryonic development; Rat; Rodentia; Central nervous system; Molecular interaction; Vertebrata; Mammalia; Cell line; Neuron; Molecular association; Cytoskeleton; Microtubule associated protein; Microtubule; Differentiation; Brain (vertebrata)
• ISSN: 0950-1991; 1477-9129
• DOI: 10.1242/dev.116.4.1151

Microtubule-associated protein 2c (MAP2c) is one of a set of embryonic MAP forms that are expressed during neuronal differentiation in the developing nervous system. We have investigated its mode of action by expressing recombinant protein in non-neuronal cell lines using cell cDNA transfection techniques. At every level of expression, all the MAP2c was bound to cellular microtubules. At low MAP2c levels, the microtubules retained their normal arrangement, radiating from the centrosomal microtubule-organising centre (MTOC) but at higher levels an increasing proportion of microtubules occurred independently of the MTOC. In most cells, radially oriented microtubules still attached to the MTOC co-existed with detached microtubules, suggesting that the primary effect of MAP2 is to increase the probability that tubulin polymerisation will occur independently of the MTOC. The MTOC-independent microtubules formed bundles whose distribution depended on their length in relation to the diameter of the transfected cell. Short bundles were attached to the cell cortex at one end and followed a straight course through the cytoplasm, whereas longer bundles followed a curved path around the periphery of the cell. By comparing these patterns to those produced by two chemical agents that stabilise microtubules, taxol and dimethyl sulphoxide, we conclude that effects of MAP2c arise from two sources. It stabilises microtubules without providing assembly initiation sites and as a result produces relatively few, long microtubule bundles. These bend only when they encounter the restraining influence of the cortical cytoskeleton of the cell, indicating that MAP2c also imparts stiffness to them. By conferring these properties of stability and stiffness to neuronal microtubules MAP2c contributes to supporting the structure of developing neurites.