Steric analysis of glycerophospholipids by circular dichroism. Stereospecifity of phospholipase D catalyzed transesterification

The circular dichroism spectra of natural glycerophospholipids and synthetic 1- sn -phosphatidic acid were recorded. 3- sn -phosphatidic acid derivatives were found to show a positive Cotton effect, while 1- sn -phosphatidic acid revealed a negative Cotton effect. The results are interpreted in term...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 66; no. 2; pp. 755 - 762
Main Authors Batrakov, S.G., Panosyan, A.G., Kogan, G.A., Bergelson, L.D.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 16.09.1975
Subjects
Circular Dichroism
Egg Yolk
Female
Glycerides
Molecular Conformation
Phosphatidylcholines
Phospholipases - metabolism
Phospholipids
Plants - enzymology
Structure-Activity Relationship
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Summary:The circular dichroism spectra of natural glycerophospholipids and synthetic 1- sn -phosphatidic acid were recorded. 3- sn -phosphatidic acid derivatives were found to show a positive Cotton effect, while 1- sn -phosphatidic acid revealed a negative Cotton effect. The results are interpreted in terms of the carboxyl sector rule. By this method phospholipase D was shown to produce stereospecifically 3- sn -phosphatidyl-1- sn -glycerol when incubated with egg yolk lecithin and exess of glycerol.
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ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(75)90574-4