Polyribosome metabolism in Escherichia coli starved for an amino acid

Most polyribosomes are inferred to be inert in starving cells, but some are in a dynamic state, since (1) messenger RNA continues to enter polyribosomes; (2) about 20 to 40% of the polyribosomes are labile after rifampycin addition; (3) β-galactosidase can be induced with a lag period no more than t...

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Published inJournal of molecular biology Vol. 62; no. 3; pp. 525 - 535
Main Authors Gurgo, Corrado, Craig, Elizabeth, Schlessinger, David, Afolayan, Adeyinka
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 28.12.1971
Subjects
Amino Acids - metabolism
Bacterial Proteins - biosynthesis
Carbon Isotopes
Cell Division
Centrifugation, Zonal
Escherichia coli - metabolism
Galactosidases
Methionine
Nucleic Acid Hybridization
Ribosomes - metabolism
Rifampin
RNA, Ribosomal - metabolism
Tritium
Uracil - metabolism
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Summary:Most polyribosomes are inferred to be inert in starving cells, but some are in a dynamic state, since (1) messenger RNA continues to enter polyribosomes; (2) about 20 to 40% of the polyribosomes are labile after rifampycin addition; (3) β-galactosidase can be induced with a lag period no more than three times as long as in growing cells; and (4) the apparent rate of synthesis of protein chains, judged by the distribution of pulse-labeled protein between ribosomes and soluble protein, is about half that in growing cells.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-2836
1089-8638
DOI:10.1016/0022-2836(71)90153-7