Appearance of a kidney-specific ribosomal protein during mouse embryonic development

Ribosomes were prepared from adult mouse liver and kidney and the protein components examined by SDS polyacrylamide gel electrophoresis. A kidney specific protein was identified and was found to be associated with the large ribosomal subunit. In vitro labelling of 11- and 14-day embryonic kidneys an...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 61; no. 1; pp. 134 - 141
Main Authors Kaulenas, M.S., Unsworth, B.R.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 06.11.1974
Subjects
Aging
Animals
Centrifugation, Density Gradient
Electrophoresis, Polyacrylamide Gel
Female
Gestational Age
Kidney - embryology
Kidney - metabolism
Liver - embryology
Liver - metabolism
Methionine - metabolism
Mice
Organ Specificity
Pregnancy
Protein Biosynthesis
Ribosomes - metabolism
Sulfur Radioisotopes
Tritium
Ultracentrifugation
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Summary:Ribosomes were prepared from adult mouse liver and kidney and the protein components examined by SDS polyacrylamide gel electrophoresis. A kidney specific protein was identified and was found to be associated with the large ribosomal subunit. In vitro labelling of 11- and 14-day embryonic kidneys and subsequent analysis of the ribosomal proteins indicated a change in the ribosomal population during development. The kidney specific protein was synthesized during the first four days of kidney organogenesis.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(74)90544-0