Stabilization of heterogeneous nuclear RNA by intercalating drugs

The effect of the intercalating drugs proflavine, ethidium and daunomycin on the rate of degradation of 14C-labeled heterogeneous nuclear RNA (HnRNA) in KB cells was studied. All three drugs decreased the rate of degradation of 14C-HnRNA to acid soluble products. The most striking effect was produce...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 52; no. 3; pp. 928 - 934
Main Authors Brinker, Jane M., Madore, H.Paul, Bello, Leonard J.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 08.06.1973
Subjects
Acridines - pharmacology
Amines - pharmacology
Binding Sites
Carbon Isotopes
Carcinoma
Cell Line
Cycloheximide - pharmacology
Dactinomycin - pharmacology
Daunorubicin - pharmacology
Drug Stability
Ethidium - pharmacology
Humans
Kinetics
Mouth Neoplasms
Nucleic Acid Conformation
Nucleic Acid Denaturation
Phenanthridines - pharmacology
RNA, Neoplasm - metabolism
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Summary:The effect of the intercalating drugs proflavine, ethidium and daunomycin on the rate of degradation of 14C-labeled heterogeneous nuclear RNA (HnRNA) in KB cells was studied. All three drugs decreased the rate of degradation of 14C-HnRNA to acid soluble products. The most striking effect was produced by proflavine which promptly and completely stabilized 14C-HnRNA against degradation. Ethidium also produced complete stabilization after a delay of 30 to 60 min. Daunomycin decreased the rate of 14C-HnRNA degradation but did not alter the fraction of 14C-HnRNA which was ultimately degraded. The results are consistent with the view that base-paired sequences are present in HnRNA in vivo and play a role in the processing of HnRNA.
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ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(73)91026-7