The use of cellulose acetate for the electrophoretic separation and quantitation of serum lactic dehydrogenase isozymes in normal and pathologic states

• Published in: Clinica chimica acta Vol. 14; no. 5; pp. 689 - 697
• Main Authors: Mager, M., Blatt, W.F., Abelmann, W.H.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.1966
• Subjects: Acetates; Adult; Aged; Cellulose; Diagnosis, Differential; Electrophoresis; Female; Humans; Isoenzymes; L-Lactate Dehydrogenase - blood; Liver Diseases - blood; Male; Middle Aged; Myocardial Infarction - blood; Pneumonia - blood; Pulmonary Embolism - blood; Staining and Labeling
• ISSN: 0009-8981; 1873-3492
• DOI: 10.1016/0009-8981(66)90196-3

Normal and abnormal lactic dehydrogenase (LDH) isozyme distribution in serum was studied using cellulose acetate electrophoresis with a p-iodonitro tetrazolium violet (INT) stain, and a graphing procedure devised to permit rapid visualization and evaluation of the electrophoretograms. This diagnostic technique was validated by employing the system to analyze sera from 7 patients with acute myocardial infarct and 7 patients with parenchymatous liver disease. In each case elevation was noted in the appropriate tissue specific isozyme (LDH 1 and LDH 5 respectively). LDH isozyme patterns in 5 of 7 patients with pneumonia showed a modest elevation of LDH 1. Serial LDH isozyme patterns of 14 patients with acute pulmonary embolism, in 12 of whom total LDH was elevated, displayed a normal distribution. It is concluded that if tissue-specific LDH isozymes are released by the lung after embolization, their serum concentrations do not reach diagnostically useful levels.