Direct evaluation of drug resistance parameters in gonococcus

We carried out complex genetic analysis of clinical samples containing N. gonorrhoeae DNA, the genotype and profile of drug resistance of this agent were evaluated. Changes in genes responsible for the formation of N. gonorrhoeae resistance to penicillins, fluoroquinolones, and spectinomycin were de...

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Published inBulletin of experimental biology and medicine Vol. 144; no. 2; pp. 227 - 230
Main Authors Il'ina, E N, Malakhova, M V, Vereshchagin, V A, Govorun, V M, Priputnevich, T V, Kubanova, A A
Format Journal Article
LanguageEnglish
Published United States Springer Nature B.V 01.08.2007
Subjects
DNA, Bacterial - genetics
Drug Resistance, Bacterial - genetics
Female
Fluoroquinolones - pharmacology
Genes, Bacterial
Gonorrhea - drug therapy
Gonorrhea - microbiology
Humans
Male
Microbial Sensitivity Tests
Mutation
Neisseria gonorrhoeae - drug effects
Neisseria gonorrhoeae - genetics
Penicillin Resistance - genetics
Sequence Analysis, DNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:We carried out complex genetic analysis of clinical samples containing N. gonorrhoeae DNA, the genotype and profile of drug resistance of this agent were evaluated. Changes in genes responsible for the formation of N. gonorrhoeae resistance to penicillins, fluoroquinolones, and spectinomycin were detected during minisequencing with subsequent MALDI-TOF mass spectrometry. The sensitivity of gonococcus was evaluated directly in the clinical sample without culturing.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-007-0296-3