The murine bone marrow macrophage, a sensitive indicator cell for murine migration inhibitory factor and a new method for their harvest

Murine bone marrow macrophages grown on Teflon-coated petri dishes for a period of 8–16 days can be removed with a yield of 90–95% and a viability greater than 95% following incubation in 1 m M EDTA. Bone marrow cells cultured on Teflon-coated dishes did not differ in their replication rate, peroxid...

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Published inCellular immunology Vol. 53; no. 2; pp. 257 - 266
Main Authors Klimetzek, Volker, Remold, Heinz G.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Inc 01.08.1980
Subjects
Animals
Bone Marrow Cells
Cell Adhesion
Cells, Cultured
Macrophage Migration-Inhibitory Factors - pharmacology
Macrophages - immunology
Mice
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Summary:Murine bone marrow macrophages grown on Teflon-coated petri dishes for a period of 8–16 days can be removed with a yield of 90–95% and a viability greater than 95% following incubation in 1 m M EDTA. Bone marrow cells cultured on Teflon-coated dishes did not differ in their replication rate, peroxidase and nonspecific esterase content, pinocytosis, secretion of lysozyme and neutral proteinases from bone marrow cells cultured on plastic dishes. Murine bone marrow macrophages were found to be sensitive indicator cells for mouse migration inhibitory factor (MIF). Large numbers of cells for the MIF assay can be obtained, since their yield is 10–15 times higher than the yield of oil-induced peritoneal exudate macrophages from the same number of mice.
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ISSN:0008-8749
1090-2163
DOI:10.1016/0008-8749(80)90327-5